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1. #T cell functional assay driver
2. #T cell functional assay Activator
3. #T cell functional assay full
4. #T cell functional assay software

We detail a protocol for concurrent assessment of multiple levels of T cell functional modulation upon GITR engagement, including T cell priming, activation and effector function, in a single assay. We apply this assay to investigate the agonist activity of the T cell co-stimulatory molecule glucocorticoid-induced TNFR-related protein (GITR). Here, we describe an optimized version of T cell suppression assay designed to test the effect of immunomodulatory agents on T cell function and activation. Standardization of optimized in vitro assays for efficient assessment of immune function of such new drugs is thus needed to facilitate clinical development of the optimal drug candidates. Together with standardized protocols and cloud-based Agilent Seahorse Analytics software, the XF Hu T Cell Activation Assay Kit provides a robust measure of T cell activation with new insights.The recent clinical success of cancer immunotherapy with checkpoint blockade has led to renewed interest into the development of immune modulatory agents with the capacity to activate anti-tumor T cell responses. Co-engagement of these receptors on the cell surface leads to intracellular signaling events and the activation of nuclear transcription factors such as Nuclear Factor of Activated T cells (NFAT), NF-kB and AP-1. It is easy-to-use and compatible with specially designed injection ports on Agilent Seahorse XF cartridges, reducing assay preparation time and improving assay reliability. We analyzed the specific surface markers of T cell function CD25 and CD95 at different time points after CPBS using a flow cytometric whole blood assay. T cell activation is initiated by engagement of the T cell antigen receptor (TCR)/CD3 complex and the co-stimulatory receptor CD28.

#T cell functional assay Activator

The ImmunoCult Human CD3/CD28 T Cell Activator from STEMCELL Technologies is a soluble tetrameric complex. The kit contains Agilent-validated ImmunoCult Human CD3/CD28 T Cell Activator and 2-deoxy-D-glucose (2-DG) and is an optimal solution delivered by Agilent and STEMCELL Technologies partnership for interrogating and modulating T cell activation on the Seahorse XF platform.

#T cell functional assay driver

Such principle is supported by recent advances in the field of immunology that recognize cellular metabolism as a primary driver and regulator of immune cell function and lineage commitment. This is accomplished via measuring proton efflux rates (PER) associated with glycolytic pathway-dependent energy production, and is built on the principle that T cell activation is accompanied by a rapid switch in cellular energy production and intermediate demand to support effective proliferation, size expansion, and differentiation. Support Services, Agreements & TrainingĪgilent Seahorse XF Hu T Cell Activation Assay Kit measures human (Hu) T cell activation response within several minutes of stimulation using Seahorse XF Analyzers.

#T cell functional assay full

Shorten the time it takes to start seeing the full value of your instrument investment Instrument & equipment deinstallation, transportation, and reinstallationĬrossLab Connect services use laboratory data to improve control and decision-makingĪdvance lab operations with lab-wide services, asset management, relocation Maintain, optimize, implement, or develop methods on new/existing Agilent solutions Instrument/software qualifications, consulting, and data integrity validations

#T cell functional assay software

Software to manage instrument access/usage, sample processing, inventories, and more Service Plans, On Demand Repair, Preventive Maintenance, and Service Center Repair